eISSN: 2221-6197 DOI: 10.31301/2221-6197

Assessment of microbiological quality of Belorussian nectar honeys

Year: 2016

Pages: 40-47

Number: Volume 8, issue 1

Type: scientific article

Summary:

The main objective of the research was to evaluate the microbiological quality of 10 honeys from Belorus collected from beekeepers in the area of Grodno. The study was conducted in 2013 at the Apiculture Division of Warsaw University of Life Sciences-SGGW. The aim of the research was the count of total number of microorganisms, count of the genus Bacillus spp., and also yeasts and molds. An assessment of microbial count in honey was made with the use of three methods: submerged culture, surface culture and spiral plate to select the best of them. Furthermore were tested selected physicochemical properties such as pH, water content and water activity. The presence of Bacillus spp., yeasts and molds, and the total number of microorganisms was found in all honeys. It was also ascertained that the appropriate method for testing the microbiological quality of honey is the classic submerged method. Tested honeys proved to be a good value. Most of the studied parameters was in the standards or were on the borders. All honeys were characterized by low pH (range 3.08-3.42), while the water activity (0.622 - 0.538 aw) and a water content (16.7-21.7%) was adequate to hinder the microbial growth.

Keywords:

Belorussian honey, microbiological quality

References:

  1. Arszułowicz A., Jakość miodu // Pszczelarstwo. V.10. P.16-18.
  2. Azeredo L. da C., Azeredo M. A. A., de Souza S. R., Dutra V., M., L. Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins // Food Chemistry. 2003. V. 80. P. 249-254.
  3. Bornus L. Miód pszczeli od producenta do konsumenta. Poznań PWRiL, 1986. 112
  4. Broadhurts C. L. Stosujemy miód. Wydawnictwo Źródła życia, 2012. 76
  5. Buzzini P. Grape must: alternative uses for the organic acid production (citric, fumaric, gluconic) //Annali della Facolta di Agraria. 1994. V. 45. P. 293-306.
  6. Chirife J., Zamora M. C., Motto A. The correlation between water activity and % moisture in honey. Fundamential aspects and application to Argentine honeys // Journal of Food Engeneering. V. 72. P. 287-292.
  7. Cymanow P. Wpływ akcesji Polski do UE na jakość handlową wybranych grup artykułów żywnościowych. Zeszyty Naukowe Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie // Problemy Rolnictwa Światowego. V. 9. P.46-49.
  8. Council Directive 2001/110 relating to honey. Official Journal of the European Communities. 2001.
  9. Gomes S., Dias L. G., Moreira L. L., Rodrigues P., Estevinho L. Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal // Food and Chemical Toxicology. 2010. V. 48. P. 544-548.
  10. ISO 7218:2007/A1. Microbiology of food anf feedingstuffs. Grneral requirements and guidance for microbial examinations. International Standard Organization. Switzerland. 2013.
  11. ISO 21527-2. Microbiology of Food and Animal Feeding Stuffs- Horizontal Method for the Enumeration of Yeasts and Moulds - Part 2: Colony Count Technique in Products with Water Activity Less Than Equal to 0.95. International Standard Organization. Switzerland. 2008.
  12. Iurlina M. O., Saiz A., Fuselli S. R., Friz R. Prevalence of Bacillus In different food products collected in Argentina, LTW. 2006. V. 39.P. 105-110.
  13. Janowicz M. Adsorpcja pary wodnej przez ciastka biszkoptowe wielowarstwowe // Inżynieria Rolnicza. 2007.P. 210
  14. Kędzia B., Hołderna- Kędzia E. Miód. Skład i właściwości biologiczne. Warszawa. Przedsiębiorstwo Wydawnicze Rzeczpospolita SA, 2008. 83
  15. Kędzia B. Miód jako źródło botulizmu niemowląt- Botulizm i miód, cz.II // Pasieka. 2009. V.1.P.10-11.
  16. Mika B., Tynka B., Ziemblińska- Buczyńska A. Porównanie bakteriobójczego działania różnych typów miodów i propolisu na bakterie gramdodatnie i gram ujemne // Podstawy biotechnologii środowiskowej - trendy, badania, implementacje. Praca zbiorowa. T. 6. Pod red. Grzegorza Cemy. Gliwice: Katedra Biotechnologii Środowiskowej. Politechnika Śląska. 2014. V. 14. P. 79-89.
  17. Midura T.F., Snowden S., Wood R.M., Arnon S.S. Isolation of Clostridium botulinum from honey // Journal of Clinical Microbiology. 1979.V. 9. P.25.
  18. Nevas M., Lindstrom M., Hautamaki K., Puoskari S., Korkeala H.: Prevalence and diversity of Clostridium botulinum types A, B, E and F in honey produced in the Nordic countries // International Journal of Food Microbiology, 2005, V.105, P.145-151.
  19. Różańska H. Microbiological quality of Polish honey // Bulletin of the Veterinary Institute in Pulawy. 2011. V. 55. P. 443-445.
  20. Szczęsna T., Rybak-Chmielewska H., Waś E., Kachaniuk K., Teper D. Characteristics of Polish unifloral honey. I. Rape honey (Brassica napus var. oleifera Metzger) // Journal of Apicultural Science. 2011. V. 55(1), P. 111-119.
  21. Semkiw P., Skowronek W., Skubida P. Changes in water content of honey during ripening under controlled condition // Journal of Apicultural Science. V.2008.V. 52(1). P. 57-63.
  22. Snowdown J.A., Cliver D.O. Microorganisms in honey // International Journal of Food Microbiology. 1996. V. 31.P 48-55.
  23. Tornuk F., Karaman S., Ozturk I., Toker O. S., Tastemus B., Sagdic O., Dogan M., Kayacier A. Quality characterization of artisanal and retail Turkish blossom honeys: Determination of physicochemical, microbiological, bioactive properties and aroma profile // Industrial Crops and Products.2013. V. 46. P. 124-131.
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eISSN: 2221-6197 DOI: 10.31301/2221-6197