Biomics

Bacterial diversity was assessed by sequencing SSU 16S rRNA gene fragments (V3-V4 hyper-variable region) obtained from the metagenomic DNA extracted from the rhizosphere soil of the two pine species (Pinus sibirica Du Tour and Pinus koraiensis Sieboldd et Zucc.) in the long-term field provenance experiment in Krasnoyarsk region (Russia). Both pines’ rhizosphere was dominated by Bradyrhizobium and Acidobacteria (subdivisions 1 and 2) genera, together contributing 30% into the total sequences. Alphaproteobacteria, Actinobacteria and Spartobacteria classes constituted 32%, 16% and 5%, respectively. Proteobacteria and Acidobacteria were the most abundant phyla in pine rhizosphere soil with 45% and 21% of the total sequences, respectively. Bacterial genera richness and evenness were higher under Siberian pine. PCA revealed that increased sequence abundance of Acidobacteria (subdivisions 16 and 6), Gaiella and Spartobacteria genera was mostly associated with Siberian pine, while  Acidobacteria (subdivisions 1, 2, 3) and Bradyrhizobium  were mostly related to Korean pine. Pine species affected (P≤0.05) the abundance of one major (Acidobacteria subdivision 16) and several minor (Acidobacteria subdivision 10, Actinomadura, Marmoricola, Iamia and Methylocella) genera. The findings can help focus future research on specific bacterial guilds in order to find how they influence desirable traits of pine trees, thus contributing to forest replanting technologies.

bacterial 16S DNA diversity; Illumina® MiSeq; rhizosphere; grey soil, Pinus sibirica, Pinus koraiensis, long-term field provenance experiment, the Western Sayans

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