Year: 2014
Pages: 155-166
Number: Volume 6, issue 3
Type: scientific article
A variety of methods is discussed for random DNA fragmentation by physical factors, chemical agents, enzymes and enzyme mixes and by PCR with nonspecific primers. Huge attention is paid to various commercial DNA fragmentation devices and kits for a full-genomic sequencing. The historical background of DNA fragmentation is briefly considered. The quoted literature, mostly methodical, covers more than 70 years period.
DNA, fragmentation, genome, sequencing, whole genome sequencing, hydrodynamic shock, cavitaion, nebulizing, ultrasound, PCR, DNase, transposase, dsDNA Fragmentase, Nextera, MuSeek