eISSN: 2221-6197 DOI: 10.31301/2221-6197

Diversity of the methods for obtaining random fragmented DNA

Year: 2014

Pages: 155-166

Number: Volume 6, issue 3

Type: scientific article

Summary:

A variety of methods is discussed for random DNA fragmentation by physical factors, chemical agents, enzymes and enzyme mixes and by PCR with nonspecific primers. Huge attention is  paid to various commercial DNA fragmentation devices and kits for a full-genomic sequencing. The historical background of DNA fragmentation is briefly considered. The quoted literature, mostly methodical, covers more than 70 years period.

Keywords:

DNA, fragmentation, genome, sequencing, whole genome sequencing, hydrodynamic shock, cavitaion, nebulizing, ultrasound, PCR, DNase, transposase, dsDNA Fragmentase, Nextera, MuSeek

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eISSN: 2221-6197 DOI: 10.31301/2221-6197