Biomics

Immunochemical staining of root cross sections of barley plants experiencing osmotic stress was carried out with the help of antibodies against peptides with the sequence of amino acids corresponding to N-region of aquaporins from the family of HvPIP2 (anti-HvPIP2). Preliminary experiments have shown the absence of cross-reactivity and specificity of binding of antibodies against peptides of HvPIP2;1 and HvPIP2;5 aquaporins towards proteins recovered from oocytes expressing cRNA of corresponding gene fragments. There was revealed a low level of staining of aquaporins at the beginning of the action of water deficit caused by osmotic stress and increased immunostaining after prolonged incubation on PEG (polyethylene glycol). Changes in aquaporin abundance were related to modulation of hydraulic conductance.

Hordeum vulgare L, water deficit, plasmalemma aquaporins, immunolocalization

Веселов С.Ю., Вальке Р., Ван Онкелен Х., Кудоярова Г.Р. Содержание и локализация цитокининов в листьях исходных и трансгенных растений табака // Физиология растений. 1999. Т. 46. С. 326–335. 

2. Кудоярова Г.Р., Холодова В.П., Веселов Д.С. Современное состояние проблемы водного баланса растений при дефиците воды // Физиология растений. 2013. Т. 60. С. 165–175.
3. Шарипова Г.В., Веселова С.В., Веселов Д.С. Динамика показателей водного обмена у растений ячменя на фоне умеренного осмотического стресса // Биомика/BIOMICS. 2013. Т. 5. C. 130-135.
4. Aroca R., Porcel R., Ruiz-Lozano J.M. 2012. Regulation of root water uptake under abiotic stress conditions // Journal of Experimental Botany. V. 63. P. 43–57.
5. Chaumont F., Barrieu F., Wojcik E., Chrispeels M.J., Jung R. Aquaporins constitutes a large and highly divergent protein family in maize // Plant Physiology. 2001. V. 125. P. 1206–1215.
6. Gomes., D. , Agasse A., Thiebaud P., Delrot S., Gerós H., Chaumont F. Aquaporins are multifunctional water and solute transporters highly divergent in living organisms // Biochimica et Biophysica Acta, Biomembranes. 2009. V. 1788. P. 1213–1228.
7. Hachez C., Veselov D., Ye Q., Reinhardt H., Knipfer T., Fricke W., Chamout F. Short-term control of maize cell and root water permeability through plasma membrane aquaporin isoforms // Plant, Cell and Environment. 2012. V. 35. P. 185–198.
8. Katsuhara M., Akiyama Y., Koshio K., Shibasaka M., Kasamo K. Functional analysis of water channels in barley roots // Plant Cell Physiology. 2002. V. 43. P. 885-893.
9. Lopez F., Bousser A., Sissoeff I., Gaspar M., Lachaise B., et al. Diurnal regulation of water transport and aquaporin gene expression in maize roots: contribution of PIP2 proteins // Plant Cell Physiology. 2003. V. 44. P. 1384–1395.
10. Maurel C., Reizer J., Schroeder J.I., Chrispeels M.J. The vacuolar membrane protein a-TIP creates water specific channels in Xenopus oocytes // EMBO Journal. 1993. V. 12. P. 2241–2247.
11. Zhu C., Schraut D., Hartung W., Schaffner A.R. Differential responses of maize MIP genes to salt stress and ABA // Journal of Experimental Botany. 2005. V. 56. P. 2971–2981.