eISSN: 2221-6197 DOI: 10.31301/2221-6197

Уровень физиологической реакции на грибок известкового расплода Ascosphaera apis не зависит от генотипов линий медоносной пчелы

Год: 2016

Страницы: 1-19

Номер: Том 8, № 1

Тип: научная статья

Рубрика: Статьи

Авторы: Аронштейн К. , Колби Д, Бойкин Д.

Аннотация:

Селекция медоносных пчел (Apis mellifera) на увеличение физиологической устойчивости к болезням является весьма желательным и экологически безопасным подходом для увеличения выживаемости семей. Отбор желательных признаков является важнейшим элементом любой программы по разведению.

В этой работе мы изучаем, зависит ли уровень физиологической реакции на грибок известкового расплода Ascosphaera apis, одного из главных грибковых патогенов пчел, от генотипа линий медоносной пчелы. Уровень устойчивости расплода к системным микозам, вызываемых грибками, определялся по выживаемости личинок под воздействием патогена in vitro. Результаты наших исследований не обнаружили статистически значимых различий в индивидуальной устойчивости к аскосферозу среди трех линий пчел (Русская, Краинская, Устойчивая К Варроатозу).

Кроме того, активация классического иммунитета и клеточных реакций на стресс не влияет на выживание инфицированных особей, что свидетельствует о том, что активация отдельных физиологических реакций, возможно, не является основным механизмом защиты против грибковых заболеваний. Поведенческие реакции взрослых особей пчел, как публиковалось ранее, вероятнее всего, являются первичными механизмами, обеспечивающими устойчивость к аскосферозу на уровне пчелиной семьи.

Ключевые слова:

медоносная пчела, Ascosphaera apis, грибковый патоген, естественный иммунитет, устойчивость

Библиографический список:

  1. Agaisse H., Perrimon N. The roles of JAK/STAT signaling in Drosophila immune responses // Immunological Reviews. 2004. V. 198. P. 72-82.
  2. Alghamdi A., Dalton L., Phillis A., E. R., Mallon E.B. Immune response impairs learning in free- flying bumble-bees // Biol. Lett. 2008. V. 4. P. 479-481.
  3. Anderson D.L., Gibson N.L. New species and isolates of spore-cyst fungi (Plectomycetes: Ascosphaerales) from Australia // Australian Systematic Botany. 1998. V. 11. P. 53-72.
  4. Arechavaleta-Velasco M.E., Alcala- Escamilla K., Robles-Rios C., Tsuruda J.M., Hunt G.J. Fine-Scale Linkage Mapping Reveals a Small Set of Candidate Genes Influencing Honey Bee Grooming Behavior in Response to Varroa Mites // PLoS ONE. 2012. V. 7 (11). P. e47269.
  5. Armitage S.A.O., Thompson J.J.W., Rolff J., Siva-Jothy M.T. Examining costs of induced and constitutive immune investment in Tenebrio molitor // Journal of Evolutionary Biology. 2003. V. 16 (5). P. 1038-1044.
  6. Aronstein K., Murray K.D. Chalkbrood disease in honey bees // J. Invertebr. Pathol. 2010. V. 103. P. 20-29. 
  7. Aronstein K.A., Colby D., Holloway B. Validation of genetic markers associated with chalkbrood resistance // Trends in Entomology. 2016. V. (in print). 
  8. Bamrick J.F. Resistance to American foulbrood in honeybees. V. Comparative pathogenesis in resistant and susceptible larvae // J. Insect Pathol. 1964 V. 6. P. 284-304.
  9. Barribeau S.M., Schmid-Hempel P. Qualitatively different immune response of the bumblebee host, Bombus terrestris, to infection by different genotypes of the trypanosome gut parasite, Crithidia bombi // Infection, Genetics and Evolution. 2013. V. 20. P. 249-256.
  10. Berkey C.D., Blow N., Watnick P.I. Genetic analysis of Drosophila melanogaster susceptibility to intestinal Vibrio cholerae infection // Cellular Microbiology. 2009. V. 11 (3). P. 461-474.
  11. Chan Q.W.T., Howes C.G., Foster L.J. Quantitative comparison of caste differences in honeybee hemolymph. // Molecular and Cellular Proteomics. 2006. V. 5 (12). P. 2252-2262.
  12. Corona M., Robinson G.E. Genes of the antioxidant system of the honey bee: annotation and phylogeny // Insect Mol Biol. 2006. V. 15 (5). P. 687- 701.
  13. Cremer S., Armitage S.A.O., Schmid- Hempel P. Social Immunity // Curr. Biol. 2007. V. 17 (16). P. R693-R702.
  14. Dubovskiy I.M., Whitten M.M.A., Yaroslavtseva O.N., Greig C., Kryukov V.Y., Grizanova E.V., Mukherjee K., Vilcinskas A., Glupov V.V., Butt T.M. Can Insects Develop Resistance to Insect Pathogenic Fungi? // PLoS ONE. 2013. V. 8 (4). P. e60248.
  15. Erler S., Popp M., Lattorff H.M.G. Dynamics of Immune System Gene Expression upon Bacterial Challenge and Wounding in a Social Insect (Bombus terrestris) // PLoS ONE. 2011. V. 6 (3). P. e18126.
  16. Evans J.D., Aronstein K., Chen Y.P., Hetru C., Imler J.-L., Jiang H., Kanost M., Thompson G.J., Zou Z., Hultmark D. Immune pathways and defense mechanisms in honey bees Apis mellifera // Insect Mol Biol. 2006. V. 15. P. 645-656.
  17. Evans J.D., Pettis J.S. Colony-level impacts of immune responsiveness in honey bees, Apis mellifera // Evolution. 2005. V. 59 (10). P. 2270-2274.
  18. Even N., Devaud J.-M., Barron A.B. General Stress Responses in the Honey Bee // Insects. 2012. V. 3. P. 1271-1298.
  19. Evison S.E.F., Fazio G., Chappell P., Foley K., Jensen A.B., Hughes W.O.H. Innate expression of antimicrobial peptides does not explain genotypic diversity in resistance to fungal brood parasites in the honey bee // Apidologie. 2015. V. P. 1-10.
  20. Evison S.E.F., Fazio G., Chappell P., Foley K., Jensen A.B., Hughes. W.O.H. Host-parasite genotypic interactions in the honey bee: the dynamics of diversity // Ecol. Evol. 2013. V. 3 (7). P. 2214-2222.
  21. Galbraith D.A., Yang X., Niño E.L., Yi S., Grozinger C., Schneider D.S. Parallel Epigenomic and Transcriptomic Responses to Viral Infection in Honey Bees (Apis mellifera) // PLoS Pathogens. 2015 V. 11 (3). P. e1004713.
  22. Gätschenberger H., Azzami K., Tautz J., Beie H. Antibacterial immune competence of honey bees   (Apis mellifera) is adapted to different life stages and environmental risks // PLoS ONE. 2013. V. 8 (8). P. e66415.
  23. Gilliam M., Taber S., Lorenz B.J., Prest D.B. Factors affecting development of chalkbrood disease in colonies of honey bee, Apis mellifera, fed pollen contaminated with Ascosphaera apis // J. Invertebr. Pathol. 1988. V. 52. P. 314-325.
  24. Glinski Z., Buczek K. Response of the Apoidea to fungal infections. // Apiacta. 2003. V. 38. P. 183-189.
  25. Gregorc A., Bowen I.D. Heat-shock and histone group protein expression in honey-bee larvae infected with Paenibacillus larvae // Cell Biol. Int. 1999. V. 23. P. 211-218.
  26. Gregorc A., Bowen I.D. The histochemical characterisation of cell death in honeybee larvae midgut after treatment with Paenibacillus larvae, Amitraz and Oxytetracycline // Cell Biol. Int. 2000. V. 24. P. 319- 324.
  27. Gregorc A., Ellis J.D. Cell death localization in situ in laboratory reared honey bee (Apis mellifera L.) larvae treated with pesticides // Pesticide Biochemistry and Physiology. 2011. V. 99 (2). P. 200- 207.
  28. Harpur B.A., Chernyshova A., Soltani A., Tsvetkov N., Mahjoorighasrodashti M., Xu Z., Zayed A. No Genetic Tradeoffs between Hygienic Behaviour and Individual Innate Immunity in the Honey Bee, Apis mellifera // PLoS One. Aug;(8):. doi:. eCollection 2014. 2014 V. 27 (9). P. e104214.
  29. Holloway B.H., Sylvester A., Bourgeois L., Rinderer T.E. Association of single nucleotide polymorphisms to resistance to chalkbrood in Apis mellifera // J Apic Res. 2012. V. 51 (2). P. 154-163.
  30. Hultmark D. Drosophila immunity: Paths and patterns // Curr Opin Immunol. 2003. V. 15. P. 12- 19. 
  31. Invernizzi C., Peñagaricano F., Tomasco I.H. Intracolonial genetic variability in honeybee larval resistance to the chalkbrood and American foulbrood parasites // Insect Soc. 2009. V. 56 (3). P. 233-240.
  32. Jensen A.B., Pedersen B.V., Eilenberg J. Differential susceptibility across honey bee colonies in larval chalkbrood resistance // Apidologie. 2009. V. 40. P. 524-534.
  33. Korir J.C., Nyakoe N.K., Awinda G., Waitumbi J.N. Complement Activation by Merozoite Antigens of Plasmodium falciparum // PLoS ONE. 2014. V. 9 (8). P. e105093.
  34. Kraaijeveld A.R., Wertheim B. Costs and genomic aspects of Drosophila immunity to parasites and pathogens. (eds. J. Rolff, S. Reynolds). Oxford University Press, 2009. 187-205 pp.
  35. Lamech  L.T.,  Haynes  C.M.  The         unpredictability of prolonged activation of stress response pathways // J. Cell Biol. 2015 V. 209 (6). P. 781-787.
  36. Lazzaro B.P., Little T.J. Immunity in a variable world // Philosophical Transactions of the Royal Society B: Biological Sciences. 2009. V. 364. P. 15-26.
  37. Mallon E.B., Brockmann A., Schmid- Hempel P. Immune response inhibits associative learning in insects // Proc Biol Sci. 2003. V. 270 (1532). P. 2471-2473.
  38. McKean K.A., Yourth C.P., Lazzaro B.P., Clark A.G. The evolutionary costs of immunological maintenance and Deployment // BMC Evolutionary Biology. 2008. V. 8. P. 76.
  39. Meyling N.V., Pell J.K. Detection and avoidance of an entomopathogenic fungus by a generalist insect predator // Ecol. Entomol. 2006. V. 31 (2). P. 162-171.
  40. Min V.A., Condron B.G. An assay of behavioral plasticity in Drosophila larvae // Neurosci Methods. 2005 V. 145 (1-2). P. 63-72. 
  41. Murray K.D., Aronstein K.A., Jones W.A. A molecular diagnostic method for selected Ascosphaera species using PCR amplification of internal transcribed spacer regions of rDNA // J Apic Res. 2005. V. 44. P. 61-64.
  42. Nakano K., Mutoh T., Mabuchi I. Characterization of GTPase-activating proteins for the function of the Rho-family small GTPases in the fission yeast Schizosaccharomyces pombe // Article first published online. 2001. V. 43.    Otti O., Tragust S., Feldhaar H. Unifying external and internal immune defences // Animal Population Ecology. 2014. V. 29 (11). P. 625-634.
  43. Palmer K.A., Oldroyd B.P. Evidence for intracolonial genetic variance in resistance to American foulbrood of honey bees (Apis mellifera): further support for the parasite/pathogen hypothesis for the evolution of polyandry // Naturwissenschaften 2003. V. 90. P. 265- 268. 
  44. Qin X., Evans J.D., Aronstein K., Murray K.D., Weinstock G.M. Genome sequences of the honey bee pathogens Paenibacillus larvae and Ascosphaera apis // Insect Mol Biol. 2006. V. 15 (5). P. 715-718. 
  45. Randolt K., Gimple O., Geissendörfer J., Reinders J., Prusko C., Mueller M.J., Albert S., Tautz J., Beier H. Immune-Related Proteins Induced in the Hemolymph After Aseptic and Septic Injury Differ in Honey Bee Worker Larvae and Adults // Archives of Insect Biochemstry and Physiology. 2008. V. 69. P. 155- 167.
  46. Rothenbuhler W.C. Behaviour genetics of nest cleaning in honey bees. I. Responses of four inbred lines to disease-killed brood // Animal Behaviour. 1964. V. 12 (4). P. 578-583.
  47. Sadd B.M., Schmid-Hempel P. Ecology & evolutionary implications of specific immune responses (eds. J. Rolff, S. Reynolds). Oxford University Press, 2009. 225-240 pp.
  48. Sahar T., Reddy K.S., Bharadwaj M., Pandey A.K., Singh S., Chitnis C.E., Gaur D. Plasmodium falciparum Reticulocyte Binding-Like Homologue Protein 2 (PfRH2) Is a Key Adhesive Molecule Involved in Erythrocyte Invasion // PLoS ONE. 2011 V. 6 (2). P. e17102.
  49. Salih D.A., Brunet A. FoxO transcription factors in the maintenance of cellular homeostasis during aging // Curr. Opin. Cell Biol. 2008 V. 20. P. 126-136. 
  50. Simone-Finstrom M.D., Spivak M. Increased Resin Collection after Parasite Challenge: A Case of Self-Medication in Honey Bees? // PLoS ONE. 2012. V. 7 (3). P. e34601. 
  51. Spivak M., Reuter G.S. Resistance to American foulbrood disease by honey bee colonies, Apis mellifera, bred for hygienic behavior // Apidologie. 2001. V. 32. P. 555-565.
  52. Swanson J.A.I., Torto B., Kells S.A., Mesce K.A., Tumlinson J.H., Spivak M. Odorants that Induce Hygienic Behavior in Honeybees: Identification of Volatile Compounds in Chalkbrood-Infected Honeybee Larvae // Journal of Chemical Ecology. 2009. V. 35 (9). P. 1108-1116.
  53. Takenaka M., Inoue H., Takeshima A., Kakura T., Hori T. C. elegans Rassf homolog, rasf-1, is functionally associated with rab-39 Rab GTPase in oxidative stress response. // Genes Cells. 2013. V. 18 (3). P. 203-210.
  54. Tanji T., Hu X., Weber A.N.R., Ip Y.P. Toll and IMD Pathways Synergistically Activate an Innate Immune Response in Drosophila melanogaster. // Mol Cell Biol 2007. V. 27 (12). P. 4578-4588.
  55. Tarpy D.R. Genetic diversity within honeybee colonies prevents severe infections and promotes colony growth // Proc Biol Sci. 2002. V. Ser. B (270). P. 99-103.
  56. Tarpy D.R., Seeley T.D. Lower disease infections in honeybee (Apis mellifera) colonies headed by polyandrous vs monandrous queens // Naturwissenschaften. 2006. V. 93. P. 195-199.
  57. Vandenberg J.D., Shimanuki H. Technique for rearing worker honey bees in the laboratory. // J Apic Res. 1987. V. 26. P. 90-97. 
  58. Villani M.G., Krueger S.R., Schroeder P.C., Consolie F., Consolie N.H., Preston-Wilsey L.M., Roberts D.W. Soil Application Effects of Metarhizium anisopliae on Japanese Beetle (Coleoptera: Scarabaeidae) Behavior and Survival in Turfgrass Microcosms // Environmental Entomology. 1994. V. 23 (2). P. 502-513. 
  59. Wright G.A., Mustard J.A., Simcock N.K., Ross-Taylor A.A., McNicholas L.D., Popescu A., Marion-Poll F. Parallel Reinforcement Pathways for Conditioned Food Aversions in the Honeybee // Curr. Biol. 2010 V. 20 (24). P. 2234-2240.
  60. Yassine H., Kamareddine L., Osta M.A. The   Mosquito Melanization Response Is Implicated in Defense against the Entomopathogenic Fungus Beauveria bassiana // PLoS Pathogens. 2012 V. 8 (11). P. e1003029.
Скачать pdf
наверх
eISSN: 2221-6197 DOI: 10.31301/2221-6197